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hcasmcs cp-h167  (Procell Inc)

 
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    Procell Inc hcasmcs cp-h167
    Treatment of <t>hCASMCs</t> with Ang II increased phosphorylation levels of MLC2 and promoted hCASMC contractile activities. We treated hCASMCs with different doses of Ang II. IF was used to measure the expression level of p-MLC2 ( A , B ) to observe cytoplasmic myoneme myofilament formation in hCASMCs. We exposed hCASMCs to constant doses of Ang II (0.1 μM/mL), which were administered based on increase in hCASMCs with time. IF was used to measure p-MLC2 expression level ( C , D ). n = 5 per group. Scale bar: 75 µm. Data presented as mean ± SEM. *** p < 0.001 vs. control group. hCASMCs: human coronary artery smooth-muscle cells; Ang II: angiotensin II; MLC2: myosin II regulatory light chain; p-MLC2: phosphorylated myosin II regulatory light chain.
    Hcasmcs Cp H167, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hcasmcs+cp-h167/pmc10932290-177-0-2?v=Procell+Inc
    Average 90 stars, based on 1 article reviews
    hcasmcs cp-h167 - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Phosphorylated CPI-17 and MLC2 as Biomarkers of Coronary Artery Spasm–Induced Sudden Cardiac Death"

    Article Title: Phosphorylated CPI-17 and MLC2 as Biomarkers of Coronary Artery Spasm–Induced Sudden Cardiac Death

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms25052941

    Treatment of hCASMCs with Ang II increased phosphorylation levels of MLC2 and promoted hCASMC contractile activities. We treated hCASMCs with different doses of Ang II. IF was used to measure the expression level of p-MLC2 ( A , B ) to observe cytoplasmic myoneme myofilament formation in hCASMCs. We exposed hCASMCs to constant doses of Ang II (0.1 μM/mL), which were administered based on increase in hCASMCs with time. IF was used to measure p-MLC2 expression level ( C , D ). n = 5 per group. Scale bar: 75 µm. Data presented as mean ± SEM. *** p < 0.001 vs. control group. hCASMCs: human coronary artery smooth-muscle cells; Ang II: angiotensin II; MLC2: myosin II regulatory light chain; p-MLC2: phosphorylated myosin II regulatory light chain.
    Figure Legend Snippet: Treatment of hCASMCs with Ang II increased phosphorylation levels of MLC2 and promoted hCASMC contractile activities. We treated hCASMCs with different doses of Ang II. IF was used to measure the expression level of p-MLC2 ( A , B ) to observe cytoplasmic myoneme myofilament formation in hCASMCs. We exposed hCASMCs to constant doses of Ang II (0.1 μM/mL), which were administered based on increase in hCASMCs with time. IF was used to measure p-MLC2 expression level ( C , D ). n = 5 per group. Scale bar: 75 µm. Data presented as mean ± SEM. *** p < 0.001 vs. control group. hCASMCs: human coronary artery smooth-muscle cells; Ang II: angiotensin II; MLC2: myosin II regulatory light chain; p-MLC2: phosphorylated myosin II regulatory light chain.

    Techniques Used: Phospho-proteomics, Expressing, Control

    PKC was activated during contraction of hCASMCs in vitro ( n = 5 per group). Ang II induced redistribution of PKC in hCASMCs. ( A ) The curve represents changes in PKCα fluorescence intensity along the “a” to “b” axis. The plot profile of the intensity from “a” to “b” was performed using ImageJ software (version 1.8.0; U.S. National Institutes of Health, Bethesda, MD, USA, https://imagej.net/ij/index.html , accessed on 18 June 2023). ( B , C ) Representative IF images of PKCδ (green) and DAPI (blue) in hCASMCs. Scale bar: 75 µm. The curve represents changes in intensity along the “a” to “b” axis. The plot profile of the intensity from “a” to “b” was performed using ImageJ software. ( D , E ) Representative IF images of PKCε (green) and DAPI (blue) in hCASMCs. Scale bar: 75 µm. The curve represents changes in intensity along the “a” to “b” axis. The plot profile of the intensity from “a” to “b” was performed using ImageJ software. Data presented as mean ± SEM.
    Figure Legend Snippet: PKC was activated during contraction of hCASMCs in vitro ( n = 5 per group). Ang II induced redistribution of PKC in hCASMCs. ( A ) The curve represents changes in PKCα fluorescence intensity along the “a” to “b” axis. The plot profile of the intensity from “a” to “b” was performed using ImageJ software (version 1.8.0; U.S. National Institutes of Health, Bethesda, MD, USA, https://imagej.net/ij/index.html , accessed on 18 June 2023). ( B , C ) Representative IF images of PKCδ (green) and DAPI (blue) in hCASMCs. Scale bar: 75 µm. The curve represents changes in intensity along the “a” to “b” axis. The plot profile of the intensity from “a” to “b” was performed using ImageJ software. ( D , E ) Representative IF images of PKCε (green) and DAPI (blue) in hCASMCs. Scale bar: 75 µm. The curve represents changes in intensity along the “a” to “b” axis. The plot profile of the intensity from “a” to “b” was performed using ImageJ software. Data presented as mean ± SEM.

    Techniques Used: In Vitro, Fluorescence, Software

    PKC mediates CPI-17/MLC2 signaling-induced contraction in cultured hCASMCs. ( A ) Representative immunofluorescent images of PKCα (green) and DAPI (blue) in hCASMCs. Scale bar: 75 µm. ( B ) The 3D waterfall plot results of PKCα immunofluorescence staining. The abscissa is the length of the cell section, and the ordinate is the gray value from a to b. ( C ) Representative immunofluorescent images of p-CPI-17 (red) and DAPI (blue) in cultured hCASMCs. Scale bar: 75 µm. ( D ) The mean fluorescence intensity of p-CPI-17 per section was quantified. n = 5 per group. ( E ) Representative immunofluorescent images of p-MLC2 (red) and DAPI (blue) in cultured hCASMCs. Scale bar: 75 µm. ( F ) The mean fluorescence intensity of p-MLC2 per section was quantified. n = 5 per group. Data presented as mean ± SEM. ns, no significance. *** p < 0.001 vs. control group; ### p < 0.001 vs. Ang II group.
    Figure Legend Snippet: PKC mediates CPI-17/MLC2 signaling-induced contraction in cultured hCASMCs. ( A ) Representative immunofluorescent images of PKCα (green) and DAPI (blue) in hCASMCs. Scale bar: 75 µm. ( B ) The 3D waterfall plot results of PKCα immunofluorescence staining. The abscissa is the length of the cell section, and the ordinate is the gray value from a to b. ( C ) Representative immunofluorescent images of p-CPI-17 (red) and DAPI (blue) in cultured hCASMCs. Scale bar: 75 µm. ( D ) The mean fluorescence intensity of p-CPI-17 per section was quantified. n = 5 per group. ( E ) Representative immunofluorescent images of p-MLC2 (red) and DAPI (blue) in cultured hCASMCs. Scale bar: 75 µm. ( F ) The mean fluorescence intensity of p-MLC2 per section was quantified. n = 5 per group. Data presented as mean ± SEM. ns, no significance. *** p < 0.001 vs. control group; ### p < 0.001 vs. Ang II group.

    Techniques Used: Cell Culture, Immunofluorescence, Staining, Fluorescence, Control



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    hcasmcs cp-h167  (Procell Inc)
    90
    Procell Inc hcasmcs cp-h167
    Treatment of <t>hCASMCs</t> with Ang II increased phosphorylation levels of MLC2 and promoted hCASMC contractile activities. We treated hCASMCs with different doses of Ang II. IF was used to measure the expression level of p-MLC2 ( A , B ) to observe cytoplasmic myoneme myofilament formation in hCASMCs. We exposed hCASMCs to constant doses of Ang II (0.1 μM/mL), which were administered based on increase in hCASMCs with time. IF was used to measure p-MLC2 expression level ( C , D ). n = 5 per group. Scale bar: 75 µm. Data presented as mean ± SEM. *** p < 0.001 vs. control group. hCASMCs: human coronary artery smooth-muscle cells; Ang II: angiotensin II; MLC2: myosin II regulatory light chain; p-MLC2: phosphorylated myosin II regulatory light chain.
    Hcasmcs Cp H167, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hcasmcs+cp-h167/pmc10932290-177-0-2?v=Procell+Inc
    Average 90 stars, based on 1 article reviews
    hcasmcs cp-h167 - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Treatment of hCASMCs with Ang II increased phosphorylation levels of MLC2 and promoted hCASMC contractile activities. We treated hCASMCs with different doses of Ang II. IF was used to measure the expression level of p-MLC2 ( A , B ) to observe cytoplasmic myoneme myofilament formation in hCASMCs. We exposed hCASMCs to constant doses of Ang II (0.1 μM/mL), which were administered based on increase in hCASMCs with time. IF was used to measure p-MLC2 expression level ( C , D ). n = 5 per group. Scale bar: 75 µm. Data presented as mean ± SEM. *** p < 0.001 vs. control group. hCASMCs: human coronary artery smooth-muscle cells; Ang II: angiotensin II; MLC2: myosin II regulatory light chain; p-MLC2: phosphorylated myosin II regulatory light chain.

    Journal: International Journal of Molecular Sciences

    Article Title: Phosphorylated CPI-17 and MLC2 as Biomarkers of Coronary Artery Spasm–Induced Sudden Cardiac Death

    doi: 10.3390/ijms25052941

    Figure Lengend Snippet: Treatment of hCASMCs with Ang II increased phosphorylation levels of MLC2 and promoted hCASMC contractile activities. We treated hCASMCs with different doses of Ang II. IF was used to measure the expression level of p-MLC2 ( A , B ) to observe cytoplasmic myoneme myofilament formation in hCASMCs. We exposed hCASMCs to constant doses of Ang II (0.1 μM/mL), which were administered based on increase in hCASMCs with time. IF was used to measure p-MLC2 expression level ( C , D ). n = 5 per group. Scale bar: 75 µm. Data presented as mean ± SEM. *** p < 0.001 vs. control group. hCASMCs: human coronary artery smooth-muscle cells; Ang II: angiotensin II; MLC2: myosin II regulatory light chain; p-MLC2: phosphorylated myosin II regulatory light chain.

    Article Snippet: hCASMCs (CP-H167, Procell, Wuhan, China) were grown in hCASMCs complete medium (CM-H167, Procell, Wuhan, China) under a water-saturated atmosphere of 95% air and 5% CO 2 at 37 °C.

    Techniques: Phospho-proteomics, Expressing, Control

    PKC was activated during contraction of hCASMCs in vitro ( n = 5 per group). Ang II induced redistribution of PKC in hCASMCs. ( A ) The curve represents changes in PKCα fluorescence intensity along the “a” to “b” axis. The plot profile of the intensity from “a” to “b” was performed using ImageJ software (version 1.8.0; U.S. National Institutes of Health, Bethesda, MD, USA, https://imagej.net/ij/index.html , accessed on 18 June 2023). ( B , C ) Representative IF images of PKCδ (green) and DAPI (blue) in hCASMCs. Scale bar: 75 µm. The curve represents changes in intensity along the “a” to “b” axis. The plot profile of the intensity from “a” to “b” was performed using ImageJ software. ( D , E ) Representative IF images of PKCε (green) and DAPI (blue) in hCASMCs. Scale bar: 75 µm. The curve represents changes in intensity along the “a” to “b” axis. The plot profile of the intensity from “a” to “b” was performed using ImageJ software. Data presented as mean ± SEM.

    Journal: International Journal of Molecular Sciences

    Article Title: Phosphorylated CPI-17 and MLC2 as Biomarkers of Coronary Artery Spasm–Induced Sudden Cardiac Death

    doi: 10.3390/ijms25052941

    Figure Lengend Snippet: PKC was activated during contraction of hCASMCs in vitro ( n = 5 per group). Ang II induced redistribution of PKC in hCASMCs. ( A ) The curve represents changes in PKCα fluorescence intensity along the “a” to “b” axis. The plot profile of the intensity from “a” to “b” was performed using ImageJ software (version 1.8.0; U.S. National Institutes of Health, Bethesda, MD, USA, https://imagej.net/ij/index.html , accessed on 18 June 2023). ( B , C ) Representative IF images of PKCδ (green) and DAPI (blue) in hCASMCs. Scale bar: 75 µm. The curve represents changes in intensity along the “a” to “b” axis. The plot profile of the intensity from “a” to “b” was performed using ImageJ software. ( D , E ) Representative IF images of PKCε (green) and DAPI (blue) in hCASMCs. Scale bar: 75 µm. The curve represents changes in intensity along the “a” to “b” axis. The plot profile of the intensity from “a” to “b” was performed using ImageJ software. Data presented as mean ± SEM.

    Article Snippet: hCASMCs (CP-H167, Procell, Wuhan, China) were grown in hCASMCs complete medium (CM-H167, Procell, Wuhan, China) under a water-saturated atmosphere of 95% air and 5% CO 2 at 37 °C.

    Techniques: In Vitro, Fluorescence, Software

    PKC mediates CPI-17/MLC2 signaling-induced contraction in cultured hCASMCs. ( A ) Representative immunofluorescent images of PKCα (green) and DAPI (blue) in hCASMCs. Scale bar: 75 µm. ( B ) The 3D waterfall plot results of PKCα immunofluorescence staining. The abscissa is the length of the cell section, and the ordinate is the gray value from a to b. ( C ) Representative immunofluorescent images of p-CPI-17 (red) and DAPI (blue) in cultured hCASMCs. Scale bar: 75 µm. ( D ) The mean fluorescence intensity of p-CPI-17 per section was quantified. n = 5 per group. ( E ) Representative immunofluorescent images of p-MLC2 (red) and DAPI (blue) in cultured hCASMCs. Scale bar: 75 µm. ( F ) The mean fluorescence intensity of p-MLC2 per section was quantified. n = 5 per group. Data presented as mean ± SEM. ns, no significance. *** p < 0.001 vs. control group; ### p < 0.001 vs. Ang II group.

    Journal: International Journal of Molecular Sciences

    Article Title: Phosphorylated CPI-17 and MLC2 as Biomarkers of Coronary Artery Spasm–Induced Sudden Cardiac Death

    doi: 10.3390/ijms25052941

    Figure Lengend Snippet: PKC mediates CPI-17/MLC2 signaling-induced contraction in cultured hCASMCs. ( A ) Representative immunofluorescent images of PKCα (green) and DAPI (blue) in hCASMCs. Scale bar: 75 µm. ( B ) The 3D waterfall plot results of PKCα immunofluorescence staining. The abscissa is the length of the cell section, and the ordinate is the gray value from a to b. ( C ) Representative immunofluorescent images of p-CPI-17 (red) and DAPI (blue) in cultured hCASMCs. Scale bar: 75 µm. ( D ) The mean fluorescence intensity of p-CPI-17 per section was quantified. n = 5 per group. ( E ) Representative immunofluorescent images of p-MLC2 (red) and DAPI (blue) in cultured hCASMCs. Scale bar: 75 µm. ( F ) The mean fluorescence intensity of p-MLC2 per section was quantified. n = 5 per group. Data presented as mean ± SEM. ns, no significance. *** p < 0.001 vs. control group; ### p < 0.001 vs. Ang II group.

    Article Snippet: hCASMCs (CP-H167, Procell, Wuhan, China) were grown in hCASMCs complete medium (CM-H167, Procell, Wuhan, China) under a water-saturated atmosphere of 95% air and 5% CO 2 at 37 °C.

    Techniques: Cell Culture, Immunofluorescence, Staining, Fluorescence, Control